Thermo Fisher Scientific Granzyme A Monoclonal Antibody (GzA-3G8.5), APC, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.125 µg/test
  View 3 publications

Product Specifications

Additional Formats

• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
This GzA-3G8.5 monoclonal antibody reacts with mouse Granzyme A, the most abundantly expressed of the ten granzyme serine proteases identified in mice. Granzymes are proteins released from NK cells and cytotoxic T lymphocytes that induce death in target cells by cleavage of intracellular substrates. They play a critical role in immune defense against viruses, tumors, and intracellular bacteria.

Granzyme A activates caspase-independent cell death pathways similar to apoptosis, characterized by mitochondrial and DNA damage. It may also play a role in inflammation, as the precursor form of IL-1β (pro-IL-1β) is among its target substrates. Granzyme A shares overlapping substrate specificity with Granzyme K, which may explain the minimal decrease in cytotoxicity observed in Granzyme A-deficient CTLs.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by intracellular staining followed by flow cytometric analysis of normal mouse splenocytes. Recommended ≤0.125 µg per test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody should be titrated for optimal performance.

Optical Properties:
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells recognize and lyse specific target cells bearing nonself antigens, typically resulting from intracellular infection. The protein described here is a T cell- and NK cell-specific serine protease that functions as a key component in the lysis of target cells by CTLs and NK cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지