Thermo Fisher Scientific TCR V beta 5.1 Monoclonal Antibody (LC4), APC, eBioscience

Thermo Fisher Scientific TCR V beta 5.1 Monoclonal Antibody (LC4), APC, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test
Publications: View 1 publication

Product Specifications

Product Specific Information

This LC4 monoclonal antibody recognizes the human T cell receptor (TCR) Vβ5.1 allele.
TCR specificity is determined by Valpha, Jalpha, Vbeta, Dbeta, and Jbeta gene rearrangement.
Vβ expression has been studied in relation to superantigens, inflammation, autoimmune disease, and HIV infection.
It is also used to phenotype T cell clonality in CD3+/TCRαβ+ large granular lymphocyte leukemias.
This receptor is expressed on a subset of peripheral blood T cells and belongs to the Ig superfamily.

This monoclonal antibody has been reported to induce apoptosis and calcium flux.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells.
Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume).
Cell number range: 10⁵–10⁸ cells/test.

Optical Properties:
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Post-manufacturing filtration: 0.2 µm

Target Information

T cell receptors (TCRs) enable discrimination between foreign and self-peptides presented by MHC class II molecules, crucial for adaptive immune response.
Recognition of self-peptides is linked to autoimmune diseases and tumors.
Superantigen engagement of TCRs can cause toxic shock syndrome.
Autoantibodies to Vβ segments have been found in RA and SLE patients, potentially modulating TH1-mediated inflammation.
Most human T cells express TCR αβ with CD4 or CD8; a minority are double negative (DN).
Increased αβ DN T cells have been observed in autoimmune and immunodeficiency disorders.
γδ T cells, found mainly in epithelial tissues, exhibit limited TCR diversity and show antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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