
Thermo Fisher Scientific GFP Recombinant Rabbit Monoclonal Antibody (HL1845)
GFP 단백질을 인식하는 고특이성 Recombinant Rabbit Monoclonal Antibody. Western blot, IHC, ICC 등 다양한 응용 가능. 높은 순도(Protein A 정제)와 안정적 형광 신호 제공. 연구용으로만 사용.
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Thermo Fisher Scientific GFP Recombinant Rabbit Monoclonal Antibody (HL1845)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HL1845 |
| Immunogen | Full length GFP recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090470 |
Target Information
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms.
As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments. GFP technology has revealed new insights into the physiological activities of living cells.
GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without additional proteins, substrates, or cofactors. GFP fluorescence is stable, species-independent, and suitable for various applications, including monitoring gene expression, protein localization, protein-protein interactions (yeast two-hybrid system), and fluorescence energy transfer (FRET) experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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