Thermo Fisher Scientific HPV Type 16 E7 Recombinant Rabbit Monoclonal Antibody (HL1821)
HPV Type 16 E7 단백질을 검출하기 위한 Thermo Fisher Scientific의 재조합 단클론 항체입니다. Western blot, ELISA, Lateral Flow 등 다양한 어세이에 적용 가능하며, HEK293 세포에서 발현된 Rabbit IgG 형식입니다. 장기 보관 시 -20°C에서 안정적입니다.
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Thermo Fisher Scientific HPV Type 16 E7 Recombinant Rabbit Monoclonal Antibody (HL1821)
Applications and Tested Dilution
- Western Blot (WB): Assay-dependent
- ELISA (ELISA): Assay-dependent
- Lateral Flow (LF): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HL1821 |
| Immunogen | Full length Human Papilloma virus type 16 E7 recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090461 |
Product Specific Information
This antibody can be used in Sandwich ELISA to detect HPV Type 16 E7 using MA5-47121 as the capture antibody and MA5-50337 as the detection antibody.
Target Information
HPV E7 proteins are small, zinc-binding phosphoproteins localized in the nucleus. HPV16 E7 consists of 98 amino acids (15 kDa) and is structurally and functionally similar to the E1A protein of subgenus C adenoviruses.
The first 16 N-terminal amino acids of HPV16 E7 contain a region homologous to the conserved region 1 (CR1) of E1A, while the next domain (up to amino acid 37) corresponds to region 2 (CR2). These domains are essential for cell transformation in vitro, suggesting a similar mechanism of transformation among these viruses.
The CR2 region includes the LXCXE motif (residues 22–26), which is involved in binding to the tumor suppressor protein pRb.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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