Thermo Fisher Scientific Actin (muscle) Monoclonal Antibody (HHF35), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

HHF35

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865197

Product Specific Information

Description: The monoclonal antibody HHF35 recognizes muscle actin; specifically the alpha and gamma form found in skeletal cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa protein is a main component of the cytoskeletal structural network. The HHF35 antibody has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. The antibody stains tumors of muscle origin such as leiomyomas, leiosarcomes and rhabdomyosacromas.

The HHF35 antibody recognizes human, mouse, rat, bovine, pig, guinea pig, sheep, frog, canine and non-human primate actin.

Applications Reported: This HHF35 antibody has been reported for use in immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections and western blotting.

Applications Tested: This HHF35 antibody has been tested by immunohistochemistry on FFPE human pancreas (with IHC Antigen Retrieval Solution - Low pH (Product # 00-4955-58) and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.