Thermo Fisher Scientific Actin (muscle) Monoclonal Antibody (HHF35), eBioscience
근육 액틴(alpha, gamma)을 특이적으로 인식하는 HHF35 단클론 항체로, 골격근·심근·평활근 조직 연구에 적합. IHC, WB 등 다양한 응용 가능. 높은 순도(>90%)와 안정적 액체형 제품으로 연구용에 최적화.
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Thermo Fisher Scientific Actin (muscle) Monoclonal Antibody (HHF35), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-Dependent |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Horse, Guinea pig, Human, Mouse, Non-human primate, Pig, Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HHF35 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865197 |
Additional Formats
Product Specific Information
The monoclonal antibody HHF35 recognizes muscle actin, specifically the alpha and gamma forms found in skeletal, cardiac, and smooth muscle. No reactivity to the beta form is observed. The 42 kDa actin protein is a key component of the cytoskeletal structural network.
HHF35 has been shown to recognize actin from smooth muscle cells of the heart, bladder, uterus, gastrointestinal tract, and diaphragm. It stains tumors of muscle origin such as leiomyomas, leiomyosarcomas, and rhabdomyosarcomas.
This antibody recognizes actin from human, mouse, rat, bovine, pig, guinea pig, sheep, frog, canine, and non-human primate sources.
Applications Reported:
Used for immunohistochemical staining of frozen and formalin-fixed paraffin-embedded tissue sections and for western blotting.
Applications Tested:
Tested by immunohistochemistry on FFPE human pancreas (with IHC Antigen Retrieval Solution - Low pH, Product # 00-4955-58) and can be used at ≤5 µg/mL. Antibody should be carefully titrated for optimal performance.
Purity: >90%, determined by SDS-PAGE
Aggregation: <10%, determined by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Actin proteins are highly conserved and play critical roles in cell motility, structure, and integrity.
- Alpha actins: major constituents of contractile apparatus
- Beta and gamma actins: regulate cell motility
This actin is an alpha actin found in skeletal muscle.
Mutations in this gene are associated with nemaline myopathy type 3, congenital myopathies, and muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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