Thermo Fisher Scientific TOX Monoclonal Antibody (TXRX10), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 6 publications 6 publications

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

TXRX10

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• eFluor 660
• PE
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865199

Product Specific Information

Description: This TXRX10 monoclonal antibody reacts with human and mouse TOX, which is also known as Thymocyte selection-associated HMG box protein. This DNA-binding nuclear factor is expressed in thymocytes, T lymphocytes, NK cells, and lymphoid tissue-inducer (LTi) cells. TOX expression in thymocytes is upregulated during beta-selection and positive selection. Studies using Tox knockout mice have demonstrated a critical role for TOX in CD4^+ T cell development as a regulator of the CD4^loCD8^lo to CD4^+CD8^lo transition. Moreover, TOX has been shown to be involved in the development of NK and LTi cells. While its regulation remains unclear, TOX expression has been shown to be induced by calcineurin-mediated TCR signaling in thymocytes during positive selection.

Applications Reported: This TXRX10 antibody has been reported for use in immunoprecipitation and immunoblotting (WB).

Applications Tested: This TXRX10 antibody has been tested by immunoblotting of reduced cell lysate prepared from mouse thymocytes at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.