Thermo Fisher Scientific Phospho-c-Raf (Tyr341) Polyclonal Antibody
Phospho-c-Raf (Tyr341) 폴리클로날 항체로 인간 시료에 반응하며, Western blot과 IHC(파라핀) 실험에 적합합니다. 고순도 친화 크로마토그래피 정제, 1 mg/mL 농도의 액상 형태로 제공되며, 연구용으로 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
- Publications: View 1 publication
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:100
- Publications: View publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 341 (S-Y-Y(p)-W-E) derived from Human C-RAF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554321 |
Product Specific Information
- Suggested positive control for Western blot: Jurkat cells
- Suggested positive control for IHC: Human pancreas tissue
Target Information
Raf-1 (c-Raf) is a MAP kinase kinase kinase (MAP3K) functioning downstream of the Ras family of membrane-associated GTPases. Once activated, Raf-1 phosphorylates and activates MEK1 and MEK2, which in turn activate ERK1 and ERK2. Activated ERKs regulate gene expression involved in cell division, apoptosis, differentiation, and migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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