Thermo Fisher Scientific Phospho-Rb (Ser795) Polyclonal Antibody
Rb 단백질의 Ser795 인산화 부위를 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 사용 가능. 인간 시료에 반응하며, 고순도 친화 크로마토그래피 정제. 연구용으로 세포주 및 조직 내 Rb 인산화 상태 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 | - |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 795 (P-S-S(p)-P-L) derived from Human Rb |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554323 |
Product Specific Information
- Suggested positive control for Western blot: K562 cells
- Suggested positive control for IHC: Human breast carcinoma
- Suggested positive control for ICC/IF: HeLa cells
Target Information
Rb (Retinoblastoma protein) is a tumor suppressor nuclear phosphoprotein that binds DNA and is phosphorylated on serine and threonine residues. It forms complexes with SV40 large T antigen, adenovirus E1A, and HPV-16 E. Rb regulates transcription, and loss of its function leads to uncontrolled cell growth. Mutations or aberrations in the RB gene are associated with various cancers, including breast, colon, prostate, kidney, nasopharyngeal, and leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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