Thermo Fisher Scientific Phospho-RACGAP1 (Ser387) Polyclonal Antibody
Phospho-RACGAP1 (Ser387) 폴리클로날 항체로 인간 및 생쥐 반응성. Western blot, IHC, ICC/IF에 사용 가능. 친화 크로마토그래피로 정제된 1 mg/mL 액상 형태. 세포골격 조절 관련 RAC 단백질 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 387 (R-I-S(p)-G-C) derived from Human GTPase Activating Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554322 |
Product Specific Information
A suggested positive control for Western blot is COS7 cells.
Target Information
RAC, a small GTPase in the ras family, regulates the formation of membrane ruffles, lamellipodia, and filopodia. It is involved in cytoskeletal actin organization and transformation. The p21 activated kinases (PAKs) are direct targets of active Rac and Cdc42, which can induce the assembly of polarized cytoskeletal structures when expressed in fibroblasts. RAC1 and RAC2 are also essential components of NADPH oxidase, the enzyme responsible for generating free radicals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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