Thermo Fisher Scientific SOX2 Polyclonal Antibody
SOX2 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합합니다. 인간, 마우스, 랫, 개, 양에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 줄기세포 유지 및 발달 연구에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5 µg/mL | View 4 publications |
| Immunohistochemistry (IHC) | – | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:125–1:250 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 | View 16 publications |
| Flow Cytometry (Flow) | 1:100 | View 3 publications |
| Miscellaneous PubMed (Misc) | – | View 2 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Sheep, Rat |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1–100). [Swiss-Prot# P48431] |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2195781 |
Product Specific Information
The target sequence has 92% sequence homology with chicken, 86% with xenopus, and 71% with zebrafish.
Suggested positive controls include antigen standard for SOX2 (transient overexpression lysate), mouse brain lysate, mouse brain protein, and uterual endometrial glands.
Target Information
SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in embryonic development and cell fate determination.
It is essential for stem-cell maintenance in the central nervous system and regulates gene expression in the stomach.
The SOX2 gene lies within an intron of another gene, SOX2 overlapping transcript (SOX2OT).
SOX2 protein may act as a transcriptional activator after forming complexes with other proteins.
Mutations in SOX2 are associated with bilateral anophthalmia, optic nerve hypoplasia, and syndromic microphthalmia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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