Thermo Fisher Scientific ADFP Polyclonal Antibody
Rabbit polyclonal antibody targeting ADFP (Adipophilin), reactive with human, mouse, rat, and bovine samples. Suitable for WB, IHC, ICC/IF, and Flow applications. Affinity purified, liquid form, 1 mg/mL concentration, stored at -20°C in PBS with 0.02% ...
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 2 publications |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | View details |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 2 publications |
| Flow Cytometry (Flow) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C-terminal region (within residues 350–425) of mouse ADFP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2223607 |
Product Specific Information
The target sequence has 88% sequence homology with rat ADFP.
Target Information
Milk lipid globules from humans, cows, and rats contain a protein identified as adipocyte differentiation-related protein (ADFP). It is associated with the globule surface membrane material. This protein, previously believed to be specific to adipocytes, is a major constituent of the globule surface and is present in a detergent-insoluble complex that contains butyrophilin and xanthine oxidase.
ADFP (Adipophilin) is found in a wide range of cultured cell lines, including fibroblasts, endothelial, and epithelial cells. In tissues, expression of adipophilin is restricted to specific cell types such as:
- Lactating mammary epithelial cells
- Adrenal cortex cells
- Sertoli and Leydig cells of the male reproductive system
- Steatosis or fatty change hepatocytes in alcoholic liver cirrhosis
ADFP may serve as a marker for identifying specialized differentiated cells containing lipid droplets and for diseases associated with fat-accumulating cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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