Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Biotin
• FITC
• Functional Grade
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 700
• PE
• Request custom conjugation

Product Specific Information

Description:
The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90–110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monoclonal antibody.

Applications Reported:
The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis and immunohistochemical staining. It has also been reported in blocking of CD54 in functional studies. (For functional assays, use Functional Grade purified YN1/1.7.4, Product #16-0541).

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain. It has seven potential N-linked glycosylation sites and shares sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and serves as a receptor for Rhinovirus. It is expressed by activated endothelial cells and various immune cells including epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils. Expression increases upon inflammation and is also observed in certain carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.