Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eBioscience
CD49f (Integrin alpha 6) 단백질을 인식하는 Rat IgG2a 단일클론 항체로, 인간 및 생쥐 시료에 반응합니다. Flow Cytometry, IHC, ICC, IP 등에 사용 가능하며, 0.5 µg/test 농도로 검증되었습니다. 세포 표면 인테그린 연구 및 세포 부착 신호전달 연구에 적합합니다.
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- 14-0495-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 1 |
| Immunohistochemistry (IHC) | - | 5 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 0.5 µg/test | 47 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | - | 2 |
| Inhibition Assays (IA) | - | 3 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioGoH3 (GoH3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_891480 |
Additional Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation available
Product Specific Information
Description:
The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f (Integrin alpha 6, VLA-6 alpha chain, platelet gpIc). CD49f is a 120 kD transmembrane protein that associates with CD29 (integrin beta 1) to form VLA-6, or with CD104 (integrin beta 4) to form α6β4 complex. It is expressed on T cells, monocytes, platelets, epithelial and endothelial cells, and germinal center B cells.
This antibody cross-reacts with integrin alpha 6 on human, mouse, and bovine cells and can block integrin alpha 6 binding to laminin.
Applications Reported:
Flow cytometry, immunoprecipitation, and immunohistology of frozen tissue sections.
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.5 µg/test (in 100 µL volume; 10⁵–10⁸ cells/test). Optimize concentration for specific assay.
Purity: Greater than 90% (SDS-PAGE)
Aggregation: Less than 10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD49f (Integrin alpha-6, ITGA6):
Integrin alpha 6 is a cell-surface protein forming heterodimers with beta chains such as beta 1 (VLA-6) or beta 4 (TSP180). Integrins mediate cell adhesion and signaling between the extracellular matrix (ECM) and cytoskeleton, influencing cell shape, motility, and proliferation.
CD49f is mainly expressed in epithelial cells and plays a structural role in hemidesmosomes. Over 18 alpha and 8 beta subunits with various isoforms exist in mammals. Certain integrins also bind soluble ligands or counter-receptors like ICAMs, facilitating cell aggregation and communication.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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