Thermo Fisher Scientific CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation available

Product Specific Information

Description:
The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f (Integrin alpha 6, VLA-6 alpha chain, platelet gpIc). CD49f is a 120 kD transmembrane protein that associates with CD29 (integrin beta 1) to form VLA-6, or with CD104 (integrin beta 4) to form α6β4 complex. It is expressed on T cells, monocytes, platelets, epithelial and endothelial cells, and germinal center B cells.
This antibody cross-reacts with integrin alpha 6 on human, mouse, and bovine cells and can block integrin alpha 6 binding to laminin.

Applications Reported:
Flow cytometry, immunoprecipitation, and immunohistology of frozen tissue sections.

Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤0.5 µg/test (in 100 µL volume; 10⁵–10⁸ cells/test). Optimize concentration for specific assay.

Purity: Greater than 90% (SDS-PAGE)
Aggregation: Less than 10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD49f (Integrin alpha-6, ITGA6):
Integrin alpha 6 is a cell-surface protein forming heterodimers with beta chains such as beta 1 (VLA-6) or beta 4 (TSP180). Integrins mediate cell adhesion and signaling between the extracellular matrix (ECM) and cytoskeleton, influencing cell shape, motility, and proliferation.
CD49f is mainly expressed in epithelial cells and plays a structural role in hemidesmosomes. Over 18 alpha and 8 beta subunits with various isoforms exist in mammals. Certain integrins also bind soluble ligands or counter-receptors like ICAMs, facilitating cell aggregation and communication.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.