Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

-

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Published species

Human, Mouse, Rat

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioKAT-1 (KAT-1, KAT1)

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• APC
• Functional Grade
• PE
• Super Bright 436
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_529544

Product Specific Information

Description: The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), which is a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, and expression increases in response to inflammatory cytokines. ICAM-1 binds to LFA-1 and this interaction is required for the transendothelial migration of T cells. ICAM-1-deficient mice are viable, however the migration of leukocytes to sites of inflammation is reduced leading to impaired immune and inflammatory responses. Based on the regulated expression of ICAM-1, it has been suggested that ICAM-1 may increase leukocyte extravasation at sites of inflammation, whereas the constitutively high expression of ICAM-2 mediates leukocyte traffic into non-inflamed tissue. The eBioKAT-1 monoclonal antibody recognizes a different epitope than the YN1/1.7.4 monoclonal antibody.

Applications Reported: This eBioKAT-1 (KAT-1, KAT1) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistology staining of frozen tissue sections, and immunohistology staining of paraffin embedded tissue sections. (Please use Functional Grade purified eBioKAT-1 (KAT-1, KAT1), Product # 16-0542, in functional assays).

Applications Tested: This eBioKAT-1 (KAT-1, KAT1) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.