Thermo Fisher Scientific CD54 (ICAM-1) Monoclonal Antibody (eBioKAT-1 (KAT-1, KAT1)), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• APC
• Functional Grade
• PE
• Super Bright 436
• Request custom conjugation

Product Specific Information

The eBioKAT-1 monoclonal antibody reacts with mouse CD54 (ICAM-1), a 95 kDa member of the immunoglobulin superfamily. CD54 is expressed at low levels on leukocytes and high endothelial venules, with expression increasing in response to inflammatory cytokines. ICAM-1 binds to LFA-1, facilitating transendothelial migration of T cells. ICAM-1-deficient mice show reduced leukocyte migration and impaired immune responses.

The eBioKAT-1 antibody recognizes a different epitope from YN1/1.7.4. Reported applications include flow cytometry, immunoprecipitation, and immunohistology on frozen and paraffin-embedded sections. For functional assays, use the Functional Grade purified form (Product #16-0542).

Applications Tested:
Tested by flow cytometry on mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.

Purity: >90% by SDS-PAGE
Aggregation: <10% by HPLC
Filtration: 0.2 µm post-manufacturing filtered

Target Information

ICAM-1 (CD54) is an 85–110 kDa single-chain type I membrane glycoprotein with five immunoglobulin-like domains, a transmembrane region, and a cytoplasmic domain. It binds integrins CD11a/CD18 (LFA-1) and CD11b/CD18 (Mac-1), and serves as a receptor for Rhinovirus.

Expressed by activated endothelial and epithelial cells, fibroblasts, chondrocytes, lymphocytes, monocytes, macrophages, dendritic cells, and neutrophils. Expression increases during inflammation and is also found in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in inflammatory conditions.

For Research Use Only. Not for use in diagnostic procedures.