Thermo Fisher Scientific Phospho-Caspase 3 (Ser26) Polyclonal Antibody
Caspase 3의 Ser26 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 적합하며, 인간, 마우스, 랫트 시료에 반응. 고순도 정제 및 안정한 PBS/glycerol buffer로 장기 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CASP3 (Accession P42574), corresponding to amino acid residues around phosphorylated Ser26 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903235 |
Product Specific Information
This antibody detects endogenous levels of Caspase 3 only when phosphorylated at Ser26.
Target Information
The CASP3 gene encodes a protein that belongs to the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce large and small subunits, which dimerize to form the active enzyme. Caspase 3 cleaves and activates caspases 6, 7, and 9, and is itself processed by caspases 8, 9, and 10. It is the predominant caspase involved in cleavage of amyloid-beta 4A precursor protein, associated with neuronal death in Alzheimer’s disease. Alternative splicing results in two transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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