Thermo Fisher Scientific Phospho-ETS1 (Ser251) Polyclonal Antibody
Phospho-ETS1 (Ser251) 폴리클로날 항체로 인간, 마우스, 랫트 시료에서 인산화된 ETS1 단백질을 검출. WB, IHC, ICC/IF 등 다양한 응용 가능. 고순도 정제 및 안정한 PBS/glycerol 저장용액 제공. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ETS1 (Accession P14921), corresponding to amino acid residues around phosphorylated Ser251 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903239 |
Product Specific Information
This antibody detects endogenous levels of ETS1 only when phosphorylated at Ser251.
Target Information
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); ETS-1; EWSR2; p54) is a 441 amino acid protein and a member of the DNA-binding ETS protein family. It contains an N-terminal pointed domain, a central transactivation domain, and two auto-inhibitory domains flanked by a conserved C-terminal Ets domain. ETS1 interacts with factors such as PEBP2alphaA and p300/CBP, mediating transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase. Activated by Ras/MAPK signaling, its expression is upregulated in resting T-cells. Activity is modulated by AP-1, Sp-1, c-myb, and MafB. Sp100 activates ETS1 in nuclear bodies. It interacts with EAP1/Daxx, repressing MMP1 and BCL2 transactivation, and with STAT6 to modulate Socs-1 expression induced by IL-4. ETS1 also interacts with GATA3 to positively regulate Tax-1 dependent IL-5 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 파일명: PA5-118739_ETS1_P14921-1_Rabbit.svg, PA5-118739_ETS1_P14921-1_Rabbit_PDP.jpeg)
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