Thermo Fisher Scientific RAD51 Recombinant Rabbit Monoclonal Antibody (JM54-26)
RAD51 단백질을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 재현성을 제공합니다. Western blot, IHC, ICC, Flow cytometry, IP 등 다양한 응용에 적합합니다. 인간, 생쥐, 랫트에 반응하며, 단백질 A 정제 및 액상 형태로 제공됩니다.
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Thermo Fisher Scientific RAD51 Recombinant Rabbit Monoclonal Antibody (JM54-26)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:1,000 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JM54-26 |
| Immunogen | Synthetic peptide within Human RAD51 (aa 1–50) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at −20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2810065 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal production candidates.
Key advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recombination. It binds to single- and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 mediates homology recognition and strand exchange between homologous DNA partners, forming a joint molecule between a processed DNA break and the repair template.
Mutations in RAD51 are associated with breast cancer, mirror movements 2, and Fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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