Thermo Fisher Scientific SATB2 Recombinant Rabbit Monoclonal Antibody (JM52-44)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

1:500

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JM52-44

Immunogen

Synthetic peptide within Human SATB2 238-287 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SATB2,uniProtId:Q9UPW6-1,ncbiNodeId:9606,antigenRange:238-287,antigenLength:733,antigenImageFileName:MA5-32788_SATB2_Q9UPW6-1_Rabbit.svg,antigenImageFileNamePDP:MA5-32788_SATB2_Q9UPW6-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2810064

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

DNA-binding protein SATB2 binds to DNA at the nuclear matrix- or scaffold associated regions. It is thought to recognize the sugar-phosphate structure of double-stranded DNA. SATB2 is a transcription factor controlling nuclear gene expression by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. It also acts as a docking site for several chromatin remodeling enzymes by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. SATB2 is required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes - probably by modulating BCL11B expression. It is a repressor of Ctip2 and a regulatory determinant of corticocortical connections in the developing cerebral cortex. Mutations in the gene are associate with cleft palate isolated (CPI).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.