Thermo Fisher Scientific HLA-G Monoclonal Antibody (01G)
HLA-G 단백질의 막 결합형을 특이적으로 인식하는 Mouse IgG1 단일클론 항체입니다. 인간 시료에 반응하며, IHC, ICC/IF, Flow Cytometry, ELISA, IP 등 다양한 응용에 사용 가능합니다. 단백질 A로 정제된 액상 형태로 제공되며, 4°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | 1 publication |
| Flow Cytometry (Flow) | 2–4 µg/mL | 1 publication |
| ELISA | Assay-Dependent | - |
| Immunoprecipitation (IP) | Assay-Dependent | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 01G |
| Immunogen | HLA-B27 transgenic mice immunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11156220 |
Additional Formats
Product Specific Information
This antibody recognizes the membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms.
HLA-G is a nonclassical MHC Class I molecule expressed on trophoblast cell surfaces.
It does not cross-react with mouse or rat antigens.
Target Information
HLA-G is a member of the HLA class I heavy chain family. It forms a heterodimer with beta-2 microglobulin, with the heavy chain anchored in the membrane.
It is expressed on fetal-derived placental cells. The heavy chain (~45 kDa) gene contains 8 exons encoding leader peptide, alpha domains, transmembrane region, and cytoplasmic tail.
HLA and MHC antibodies are crucial in Immunopeptidomics, aiding neoantigen identification and characterization via LC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(관련 항원 구조 및 이미지 파일명: MA1-10359_HLA-G_P17693-1_House_mouse.svg, MA1-10359_HLA-G_P17693-1_House_mouse_PDP.jpeg)
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