Thermo Fisher Scientific HLA-G (soluble) Monoclonal Antibody (2A12)
인간 HLA-G 단백질에 특이적인 단일클론 항체로, 분비형 HLA-G5 및 HLA-G6 동형체를 구별 가능. Western blot, IHC, Flow cytometry, ELISA 등 다양한 응용에 적합. 액상 형태, 1 mg/mL 농도, 4°C 보관.
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Thermo Fisher Scientific HLA-G (soluble) Monoclonal Antibody (2A12)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Flow Cytometry (Flow) | 1–4 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2A12 |
| Immunogen | C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11156617 |
Additional Formats
Product Specific Information
This antibody was generated to a peptide corresponding to C-intron 4-encoded sequence. It does not cross-react with the full-length HLA-G1 isoform and thus allows distinction between secreted HLA-G5 and HLA-G6 isoforms from shedded HLA-G1.
Target Information
HLA-G belongs to the HLA class I heavy chain paralogues. This molecule is a heterodimer composed of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain (~45 kDa) is membrane-anchored and encoded by a gene with 8 exons:
- Exon 1: leader peptide
- Exons 2 & 3: alpha1 and alpha2 domains (peptide-binding)
- Exon 4: alpha3 domain
- Exon 5: transmembrane region
- Exon 6: cytoplasmic tail
HLA-G is expressed on fetal-derived placental cells.
HLA and MHC antibodies are critical in Immunopeptidomics, enabling identification and characterization of neoantigens via high-performance liquid chromatography and tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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