Thermo Fisher Scientific HLA-G (soluble) Monoclonal Antibody (5A6G7)

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Biotin
• PE
• Request custom conjugation

Product Specific Information

This antibody was generated against a peptide corresponding to the C-intron 4-encoded sequence. It does not cross-react with the full-length HLA-G1 isoform, allowing distinction between secreted HLA-G5 and HLA-G6 isoforms and shedded HLA-G1.

• Purity: >95% by SDS-PAGE
• ELISA Positive Control: HeLa/HLA-G5 transfectant lysate or supernatant
• ELISA Negative Control: HeLa cell lysate
• Sandwich ELISA: Capture antibody in combination with antibody W6/32 (Product # MA1-19526)
• Western Blot Positive Control: JEG-3 cell lysate (reducing conditions, 12% AA SDS-PAGE)
• Western Blot Conditions: Reducing

Target Information

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer composed of a heavy chain and a light chain (β2-microglobulin). The heavy chain (~45 kDa) is membrane-anchored and encoded by a gene with 8 exons:

• Exon 1: Leader peptide
• Exons 2–3: α1 and α2 domains (peptide-binding)
• Exon 4: α3 domain
• Exon 5: Transmembrane region
• Exon 6: Cytoplasmic tail

HLA-G is expressed on fetal-derived placental cells.
HLA and MHC antibodies are key tools in Immunopeptidomics, supporting the identification and characterization of neoantigens via LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.