Thermo Fisher Scientific Aquaporin 2 Polyclonal Antibody
Aquaporin 2 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot, IHC, ICC/IF에 사용 가능. 사람, 생쥐, 랫트 반응성. Protein A 정제, 1 mg/mL 농도. PBS (pH 7.4) + 50% glycerol 보존, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:200 |
| Immunocytochemistry (ICC/IF) | 1:60 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Produced against the C-terminal peptide (Sequence N-CLKGLEPDTDWEEREVRRRQ) of rat aquaporin 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735464 |
Product Specific Information
- 0.5 µg/mL of PA5-77841 was sufficient for detection of aquaporin 2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Detects approximately 28.8 kDa. May detect larger glycosylated bands approximately 35–50 kDa.
Target Information
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct.
Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans.
A vasopressin-induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from intracellular vesicles to the apical membrane of principal cells.
Recently, serine-261 has been identified as a novel phosphorylation site on AQP2, and levels of phosphorylated S261 decrease with vasopressin treatment, suggesting its involvement in vasopressin-dependent AQP2 trafficking.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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