Thermo Fisher Scientific Aquaporin 1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Frozen) (IHC (F))

1:200

-

Immunocytochemistry (ICC/IF)

1:400

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Produced against the N-terminal peptide (sequence N-MASEFKKKLF) of rat Aquaporin 1 (aa 1-10). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Aquaporin 1,uniProtId:P29975-1,ncbiNodeId:10114,antigenRange:1-10,antigenLength:269,antigenImageFileName:PA5-77842_Aquaporin_1_P29975-1_Rabbit.svg,antigenImageFileNamePDP:PA5-77842_Aquaporin_1_P29975-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735461

Product Specific Information

0.5 µg/mL of PA5-77842 was sufficient for detection of aquaporin 1 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 28.5kDa. May detect larger glycosylated bands approximately 35-50kDa.

Target Information

Aquaporin 1 is a 28kD integral membrane protein which was originally identified in red blood cells and renal proximal tubules. AQP1 is also expressed by the choroid plexus and various other tissues. It forms a water-specific channel that provides the plasma membranes of red cells and kidney proximal tubules with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.