Thermo Fisher Scientific ChAT Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ChAT,uniProtId:P28329-1,ncbiNodeId:9606,antigenRange:1,antigenLength:748,antigenImageFileName:PA5-77838_ChAT_P28329-1_Rabbit.svg,antigenImageFileNamePDP:PA5-77838_ChAT_P28329-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735637

Product Specific Information

A 1:1,000 dilution of PA5-77838 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.|Predicted molecular weight at approximately 82.5kDa. Observed molecular weights between 68-70kDa.

Target Information

ChAT catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.