Thermo Fisher Scientific CD41a Monoclonal Antibody (eBioMWReg30 (MWReg30)), APC, eBioscience

Applications

Immunocytochemistry (ICC/IF)

• Tested dilution: Not specified
• Publications: View 1 publication

Flow Cytometry (Flow)

• Tested dilution: 0.25 µg/test
• Publications: View 52 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 555 / 660-120S
• NovaFluor Yellow 570 / 660
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

Description:
The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha IIb). CD41 is expressed on platelets, megakaryocytes, and hematopoietic progenitors in embryos, fetuses, and adults. CD41 associates with CD61 (gpIIIa, integrin beta III) to form a receptor critical for platelet function, mediating binding to adhesion molecules such as fibrinogen, fibronectin, and vitronectin.

Recent studies using SLAM-family markers (CD48, CD150) revealed that CD150⁺CD48⁻ bone marrow cells are efficient hematopoietic stem cells (HSCs). Further enrichment by excluding CD41⁺ cells (CD150⁺CD48⁻CD41⁻) enhances reconstitution efficiency, identifying HSCs with high precision.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Tested by flow cytometric analysis of mouse platelets
• Recommended ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimize dilution for specific assays

Excitation / Emission:
633–647 nm / 660 nm (Red Laser)

Filtration:
0.2 µm post-manufacturing filtered

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B):
CD41 consists of α (120 kDa) and β (23 kDa) subunits that interact with CD61 in the presence of calcium to form a functional adhesive receptor. It mediates platelet aggregation and binds to proteins such as von Willebrand factor, fibrinogen, fibronectin, and vitronectin upon vascular injury. CD41 is mainly expressed in megakaryocyte-platelet lineages and during early hematopoietic differentiation.

Associated Diseases:

• Glanzmann Thrombasthenia
• Platelet type-16 bleeding disorder

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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