Thermo Fisher Scientific CD43 Monoclonal Antibody (4-29-5-10-21), APC, eBioscience

Thermo Fisher Scientific CD43 Monoclonal Antibody (4-29-5-10-21), APC, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells.
Use 5 µL (0.125 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser

Product Specifications

Product Specific Information

The monoclonal antibody 4-29-5-10-21 reacts with human and non-human primate CD43, also known as leukosialin, galactoglycoprotein, and sialophorin. CD43 is a sialomucin with both adhesive and anti-adhesive functions. It is expressed on most leukocytes and a subset of B lymphocytes. Activation leads to proteolytic processing and decreased surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. Its counter-receptor, CD169/SIGLEC-1, is expressed on macrophages.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with a high negative charge, expressed on most hematopoietic cells. It contributes to a repulsive barrier that interferes with adhesion but can also promote leukocyte aggregation. Through interaction with actin-binding proteins ezrin and moesin, CD43 regulates T-cell morphology and lymphocyte traffic. CD43 signaling enhances LFA-1 adhesiveness and modulates other receptor pathways. Expression of CD43 can induce tumor suppressor p53; however, in p53/ARF deficiency, CD43 promotes tumor proliferation. CD43 dysfunction is associated with Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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