Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), APC, eBioscience

Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), APC, eBioscience™

Applications

• Immunocytochemistry (ICC/IF)
  사용 농도: -
  View 1 publication

• Flow Cytometry (Flow)
  사용 농도: 5 µL (0.06 µg)/test
  View 10 publications

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 5C3 monoclonal antibody reacts with human CD40, a 45–50 kDa type I transmembrane glycoprotein. CD40, a member of the TNFR family, is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. It regulates B cell development and maturation by inducing Ig isotype-switching and, together with IL-4, protects B cells from apoptosis and promotes proliferation.

Interaction of CD40 with CD154 (gp39), its ligand on T cells, is crucial for T–B cell crosstalk and immune regulation. The 5C3 clone is reported to activate B cells in in vitro functional assays.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.06 µg) per test.
A test is defined as the amount of antibody that stains a cell sample in a 100 µL final volume.
Cell number may range from 10⁵ to 10⁸ cells/test.
Excitation: 633–647 nm
Emission: 660 nm
Laser: Red
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD40 is a single-chain glycoprotein belonging to the TNF/NGF superfamily and shows homology to CD30. It is expressed on all B cells except plasma cells, as well as on some epithelial and dendritic cells. CD40 mediates immune and inflammatory responses including T cell-dependent Ig class switching, memory B cell development, and germinal center formation.

AT-hook transcription factor AKNA regulates CD40 expression, while TNFR2 serves as a signal transduction mediator. CD40–CD154 interaction is implicated in amyloid-beta-induced microglial activation, an early event in Alzheimer’s disease pathogenesis. Two alternatively spliced CD40 transcript variants are known.

Associated Diseases:

• Type 3 Hyper-IgM Immunodeficiency
• CD40 Ligand Deficiency

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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