Thermo Fisher Scientific Phospho-NPM1 (Thr199) Polyclonal Antibody
인간, 생쥐, 랫트에서 인산화된 NPM1(Thr199)을 특이적으로 검출하는 Rabbit Polyclonal 항체입니다. Western blot, IHC, ICC/IF에 사용 가능하며, 고순도 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며 -20°C에서 보관합니다.
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Thermo Fisher Scientific Phospho-NPM1 (Thr199) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NPM1 (Accession P06748), corresponding to amino acid residues around phosphorylated Thr199 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816234 |
Product Specific Information
This antibody detects endogenous levels of Nucleophosmin only when phosphorylated at Threonine 199.
Target Information
NPM1 (Nucleophosmin 1, also known as B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in:
- Ribosome assembly and transport
- Cytoplasmic/nuclear trafficking
- Regulation of DNA polymerase alpha activity
- Centrosome duplication
- Regulation of p53 activity
NPM1 continuously shuttles between nucleus, cytoplasm, and nucleolus, chaperoning core histones. It binds nucleic acids, prevents protein aggregation, protects enzymes during thermal denaturation, and assists in renaturation of denatured proteins. NPM1 is associated with centrosomes and acts as a substrate of CDK2/cyclin E during centrosome duplication.
Due to its gene interactions with tumor-associated translocations, NPM1 forms fusion proteins such as NPM-ALK, NPM-RAR, and NPM-MLF1, contributing to oncogenic processes. It also sequesters tumor suppressor RF in the nucleolus, protecting it from degradation. Dysfunction of NPM1 is linked to diseases such as acute myeloid leukemia and lymphomatoid papulosis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
제품 이미지
(이미지 파일: PA5-104761_NPM1_P06748-1_Rabbit.svg, PA5-104761_NPM1_P06748-1_Rabbit_PDP.jpeg)
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