Thermo Fisher Scientific Collagen III Monoclonal Antibody (FH-7A)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

1:4,000

-

ELISA (ELISA)

Assay-dependent

-

Dot blot (DB)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Rabbit, Rat

Published species

Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

FH-7A

Immunogen

Human collagen type III protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4 mg/mL

Storage buffer

Ascites

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_558480

Product Specific Information

Recommended positive controls: human skin.

MA1-22147 specifically recognizes native and denatured collagen type III in human and rat samples. It does not recognize collagen types I, II, IV, V, VI and X.

By Western blot, this antibody detects a ~70 kDa band in reduced denatured samples, and a ~300 kDa band in non-reduced, non-denatured samples.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in th gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.