Thermo Fisher Scientific DNAI1 Monoclonal Antibody (70.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Product Specifications

Species Reactivity

Amphibian, Chicken, Deer, Hamster, Human, Marsupial, Mouse, Rat

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

70.1

Immunogen

Cytoplasmic, full length, native, chicken dynein intermediate chain protein purified from chick brain.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

Ascites

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2096662

Product Specific Information

Recommended positive controls: chicken brain.

This antibody is know to detect cytoplasmic Dynein intermediate chains, and reactivity with the axonemal form is not known.

By Western blot, MA1-22146 detects a ~ 70 kDa triplet representing the Dynein intermediate chains.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Eukaryotic cells rely on actin and microtubule-based protein motors to generate intracellular movements. These protein motors contain specialized domains that hydrolyze ATP to produce force and movement along a cytoskeletal polymer (actin in the case of myosin family and microtubules in the case of the kinesin family and dyneins). Dynein has been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport, the endocytic pathway, and the organization of the Golgi apparatus. In all cell types, dynein has the same basic structures and is composed of two or three distinct heavy chains (~450 kDa), three intermediate chains (70-125 kDa), and at least four light chains (15-25 kDa).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.