Thermo Fisher Scientific DNAI1 Monoclonal Antibody (70.1)
Dynein intermediate chain을 검출하는 Mouse IgM monoclonal antibody. Western blot 및 다양한 immunoassay에 사용 가능. Amphibian~Human 등 광범위한 species에 반응. 연구용으로 세포 내 운동 단백질 분석에 적합.
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Thermo Fisher Scientific DNAI1 Monoclonal Antibody (70.1)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Amphibian, Chicken, Deer, Hamster, Human, Marsupial, Mouse, Rat |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 70.1 |
| Immunogen | Cytoplasmic, full length, native, chicken dynein intermediate chain protein purified from chick brain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2096662 |
Product Specific Information
- Recommended positive control: chicken brain
- Detects cytoplasmic Dynein intermediate chains; reactivity with axonemal form not known
- By Western blot, MA1-22146 detects a ~70 kDa triplet representing Dynein intermediate chains
- Store product as a concentrated solution and centrifuge briefly prior to opening the vial
Target Information
Eukaryotic cells rely on actin and microtubule-based protein motors to generate intracellular movements. These motors hydrolyze ATP to produce force and movement along cytoskeletal polymers—actin (myosin family) or microtubules (kinesin and dynein families).
Dynein plays critical roles in cytoplasmic motile functions such as chromosomal movement, retrograde organelle and axonal transport, endocytic pathways, and Golgi apparatus organization.
Dynein structure includes two or three heavy chains (~450 kDa), three intermediate chains (70–125 kDa), and at least four light chains (15–25 kDa).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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