Thermo Fisher Scientific ATP6V1B2 Polyclonal Antibody
ATP6V1B2 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot 및 IHC(P)에서 검증됨. 인간, 마우스, 랫트에 반응하며, 고순도 항원 친화 크로마토그래피 정제. 세포 내 산성화 연구 및 단백질 발현 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V1B2 (exact sequence proprietary) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2735504 |
Product Specific Information
- Positive Control: U87-MG, SK-N-SH, IMR32, SK-N-AS, Mouse brain, Rat brain
- Predicted Reactivity: Zebrafish (96%), Xenopus laevis (97%), Chicken (99%), Chimpanzee (99%), Bovine (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATP6V1B2 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles.
V-ATPase-dependent organelle acidification is essential for processes such as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
The V1 domain of V-ATPase contains the ATP catalytic site and is composed of multiple subunits (A, B, G, C, D, E, F, H).
ATP6V1B2 is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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