Thermo Fisher Scientific SSEA5 Monoclonal Antibody (8E11-SSEA5), eBioscience

Thermo Fisher Scientific SSEA5 Monoclonal Antibody (8E11-SSEA5), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The monoclonal antibody 8E11-SSEA5 recognizes human stage-specific embryonic antigen-5 (SSEA-5), an H type-1 glycan. SSEA-5 is highly expressed on pluripotent human stem cells including embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. During embryonic development, SSEA-5 is expressed within the blastocyst on inner cell mass (ICM) cells that give rise to ES cells. As ES and iPS cells differentiate, SSEA-5 expression is downregulated.

The 8E11 antibody, in combination with either CD9 and CD90 or CD50 and CD200, can effectively separate teratoma-forming cells from stem cell-derived populations of differentiated cells.

Applications Reported: Flow cytometric analysis, Western blotting, and Immunocytochemistry.
Applications Tested:

• ICC on unfixed human NTERA-2 cells ≤10 µg/mL
• Flow cytometry on NTERA-2 cells ≤0.25 µg/test
  Careful titration is recommended for optimal assay performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Stage-Specific Embryonic Antigen-5 (SSEA-5) is an oligosaccharide found on glycosphingolipids and glycoproteins. It contains the motif Fuc1-2Gal-β1-3GlcNAc-β, characteristic of H-1 antigens. SSEA-5 is expressed on the inner cell mass of the human blastocyst, undifferentiated human ES cells, and induced pluripotent stem cells. Its expression precedes the loss of other stem cell markers. The combination of SSEA-5 with CD9/CD90 or CD50/CD200 enhances identification and elimination of rare undifferentiated ES cells from differentiated populations.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.