Thermo Fisher Scientific CD30 Monoclonal Antibody (Ber-H2), PE, eBioscience
인간 CD30 항원 인식용 Ber-H2 단일클론 항체로, PE 형광체 결합되어 유세포분석(Flow Cytometry)에 최적화. 활성화된 T/B 림프구 및 CD30+ 림프종 세포 검출에 사용. 4°C 암소 보관, 동결 금지. 연구용 한정.
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Thermo Fisher Scientific CD30 Monoclonal Antibody (Ber-H2), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.06 µg)/test
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Ber-H2 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1633388 |
Additional Formats
- APC
- FITC
- PE-Cyanine7
- Super Bright 702
- Request custom conjugation
Product Specific Information
The Ber-H2 monoclonal antibody reacts with human CD30, a 120 kDa transmembrane glycoprotein of the TNFR superfamily. CD30 is expressed on normal activated T and B lymphocytes, but not on resting lymphocytes. It is also found on certain lymphomas, embryonal carcinomas, and virus-infected lymphocytes (HIV, HTLV, EBV). Interaction with its ligand CD30L leads to signaling and activation of NF-kappaB, regulating apoptosis.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use 5 µL (0.06 µg) per test (final volume 100 µL). Cell number range: 10⁵–10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD30 (Ki-1, TNFRSF8) is a type I transmembrane glycoprotein of the TNF receptor superfamily. Originally identified on Hodgkin’s and Reed-Sternberg cells, CD30 binds CD30L (CD153) to mediate effects including proliferation, activation, differentiation, and apoptosis. It plays a key role in Hodgkin’s disease and CD30+ lymphomas, acting as a costimulatory molecule in thymic negative selection.
CD30 expression is found in:
- Hodgkin’s and Reed-Sternberg cells
- Anaplastic Large Cell Lymphomas (ALCL)
- Some non-Hodgkin’s lymphomas (e.g., Burkitt’s)
- Virus-infected T/B cells
- Activated normal T/B cells
Soluble CD30 (sCD30) serves as a Th2 immune response marker. TRAF2 and TRAF5 interact with CD30, activating NF-kappaB signaling. CD30 positively regulates apoptosis and limits autoreactive CD8 effector T cell proliferation.
Two alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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