Thermo Fisher Scientific Phospho-Caspase 8 (Tyr448) Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

Antibody detects endogenous levels of Caspase 8 only when phosphorylated at Tyr448.

Target Information

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of large and small subunits.

Caspase 8 is involved in programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain suggests interaction with FADD. Caspase 8 has been detected in insoluble fractions of affected brain regions from Huntington disease patients, implicating its role in neurodegenerative processes. It binds to the death effector domain (DED) of FADD through tandem DED domains in its pro-form, subsequently activating downstream caspases such as Caspase 9, committing the cell to apoptosis. Overexpression induces apoptosis, which can be blocked by ICE family inhibitors. Multiple alternatively spliced transcript variants encoding different isoforms are known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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