Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), NovaFluor Red 755, eBioscience

Applications and Tested Dilution

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Product Specifications

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Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available

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Product Specific Information

The 390 monoclonal antibody reacts with mouse CD31 (platelet-endothelial cell adhesion molecule-1, PECAM-1, gpIIa). This 130–140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. CD31 mediates homotypic interactions critical for adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Flow cytometric analysis and immunohistochemical staining of frozen tissue sections.

Applications Tested:

• Flow cytometry: ≤ 0.6 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Immunohistochemistry (frozen): ≤ 20 µg/mL
  Careful titration is recommended for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates use Phiton™ technology with engineered nucleic acid dye structures, providing optimized fluorescent signatures with reduced spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

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Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling through ITIM motifs that recruit protein-tyrosine phosphatases upon phosphorylation. It is widely expressed in the vascular system, mainly at endothelial junctions. The N-terminal Ig-like domain mediates homophilic binding, facilitating cell-cell interactions. CD31 plays roles in:

• Integrin-mediated adhesion
• Transendothelial migration
• Angiogenesis and apoptosis
• Negative regulation of immunoreceptor signaling
• Autoimmunity, macrophage phagocytosis, and thrombosis

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For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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