Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), NovaFluor Red 755, eBioscience

Applications

• Flow Cytometry (Flow): ≤ 0.6 µg/test
• Immunohistochemistry (Frozen Tissue): ≤ 20 µg/mL

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed on endothelial cells, leukocytes, and platelets. CD31 mediates adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported:
Suitable for flow cytometry and immunohistochemical staining of frozen tissue sections.

Applications Tested:

• Flow cytometry on mouse thymocytes and splenocytes: ≤ 0.6 µg/test (100 µL final volume)
• Immunohistochemistry (frozen tissue): ≤ 20 µg/mL
  Antibody should be titrated for optimal performance.

Important Notes:

• NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample (10³–10⁸ cells).
  • For single-color controls: 5 µL per 100 µL cell sample.

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread.

Excitation: 636 nm
Emission: 756 nm
Laser: 633–640 nm (Red)

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs. It is mainly located at endothelial junctions and mediates homophilic binding for cell-cell interactions. CD31 functions in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, and immune regulation. It plays a key role in vascular homeostasis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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