Thermo Fisher Scientific Phospho-DOK1 (Tyr362) Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human Dok-1 around the phosphorylation site of Tyrosine 362 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DOK1,uniProtId:Q99704-1,ncbiNodeId:9606,antigenRange:362,antigenLength:481,antigenImageFileName:PA5-36845_DOK1_Q99704-1_Rabbit.svg,antigenImageFileNamePDP:PA5-36845_DOK1_Q99704-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2553774

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 62 kDa.

Purity is >95% by SDS-PAGE.

Target Information

DOK1 (P62dok, Docking protein 1) is believed to be a mainly cytoplasmic adaptor protein which down-regulates mitogen-activated protein kinase activation, inhibits cell proliferation and transformation, and promotes cell spreading and cell migration. DOK1 is a major substrate for many tyrosine kinases including c-kit, v-abl, vFPS, EGF, and PDGF. Upon phosphorylation by kinases, DOK1 forms a complex with ras GTPase-activating protein. Phosphorylation on tyrosine residues by the insulin receptor kinase results in the negative regulation of the insulin signaling pathway. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.