Thermo Fisher Scientific TPM1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 92 and 273 of Human Tropomyosin 1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TPM1,uniProtId:P09493-1,ncbiNodeId:9606,antigenRange:92-273,antigenLength:284,antigenImageFileName:PA5-29846_TPM1_P09493-1_Rabbit.svg,antigenImageFileNamePDP:PA5-29846_TPM1_P09493-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.05 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547320

Product Specific Information

Recommended positive controls: A431, mouse liver, rat muscle.

Predicted reactivity: Mouse (99%), Rat (88%), Zebrafish (80%), Xenopus laevis (83%), Pig (88%), Rabbit (88%), Chicken (81%), Sheep (88%), Bovine (88%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Tropomyosin is a dimeric coiled-coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F-actin filaments, and the lack of proline and tryptophan.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.