Thermo Fisher Scientific RAP1GAP Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive controls: mouse brain, rat brain
• Predicted reactivity: Human (99%), Mouse (97%), Rat (98%), Zebrafish (81%), Bovine (97%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Rap1GAP specifically stimulates the GTP hydrolytic activity of the monomeric G protein Rap1.
Physical interaction between G alpha z (a Gi family member) and Rap1GAP blocks regulators of G protein signaling from stimulating GTP hydrolysis of the alpha subunit, and attenuates the ability of activated G alpha z to inhibit adenylyl cyclase.
Rap1GAP is expressed in the brain, kidney, and pancreas, and may act as a signal integrator coordinating Gz and Rap1 signaling.
A novel isoform, Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases GTP-bound Rap1, resulting in ERK/MAPK activation.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.