Thermo Fisher Scientific RAP1GAP Polyclonal Antibody
RAP1GAP 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat에 반응합니다. Western blot과 IHC(P)에서 검증되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein fragment corresponding to amino acids 170–473 of Human RAP1GAP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.51 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 0.1M Tris Glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547315 |
Product Specific Information
- Recommended positive controls: mouse brain, rat brain
- Predicted reactivity: Human (99%), Mouse (97%), Rat (98%), Zebrafish (81%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Rap1GAP specifically stimulates the GTP hydrolytic activity of the monomeric G protein Rap1.
Physical interaction between G alpha z (a Gi family member) and Rap1GAP blocks regulators of G protein signaling from stimulating GTP hydrolysis of the alpha subunit, and attenuates the ability of activated G alpha z to inhibit adenylyl cyclase.
Rap1GAP is expressed in the brain, kidney, and pancreas, and may act as a signal integrator coordinating Gz and Rap1 signaling.
A novel isoform, Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases GTP-bound Rap1, resulting in ERK/MAPK activation.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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