Thermo Fisher Scientific TRPC6 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive controls: NT2D1, MCF-7, A549, A549 membrane fraction extract
• Predicted reactivity: Mouse (85%), Rat (88%), Bovine (91%), Guinea pig (91%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Trpc6 forms a receptor-activated calcium channel in the cell membrane. The Trpc6 channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium.

Defects in the Trpc6 gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). Trpc6 belongs to the mammalian transient receptor potential (TRP) superfamily, specifically the “canonical TRP” (TRPC) family. Members of this family are involved in receptor-regulated cation channel activity. The TRPC3/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting activation via PLC-coupled receptors.

When expressed in transfected cells, Trpc6 acts as a non-selective, store-independent, receptor-activated cation channel. It is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that Trpc6 encodes endogenous DAG-activated receptor-operated cation channels in vivo.

Diseases associated with TRPC6 include Glomerulosclerosis, Focal Segmental, 2 and Focal Segmental Glomerulosclerosis.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.