Thermo Fisher Scientific TRPC6 Polyclonal Antibody
TRPC6 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot 및 Immunocytochemistry에 적합. 항원 친화 크로마토그래피로 정제된 액상 형태. 인간 TRPC6(aa 710–931) 유래 재조합 단백질로 면역화. 단기 4°C, 장기 -20°C 보관 권장.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TRPC6 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 710–931 of Human TRPC6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547322 |
Product Specific Information
- Recommended positive controls: NT2D1, MCF-7, A549, A549 membrane fraction extract
- Predicted reactivity: Mouse (85%), Rat (88%), Bovine (91%), Guinea pig (91%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Trpc6 forms a receptor-activated calcium channel in the cell membrane. The Trpc6 channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium.
Defects in the Trpc6 gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). Trpc6 belongs to the mammalian transient receptor potential (TRP) superfamily, specifically the “canonical TRP” (TRPC) family. Members of this family are involved in receptor-regulated cation channel activity. The TRPC3/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting activation via PLC-coupled receptors.
When expressed in transfected cells, Trpc6 acts as a non-selective, store-independent, receptor-activated cation channel. It is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that Trpc6 encodes endogenous DAG-activated receptor-operated cation channels in vivo.
Diseases associated with TRPC6 include Glomerulosclerosis, Focal Segmental, 2 and Focal Segmental Glomerulosclerosis.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.