Thermo Fisher Scientific AHA1 Monoclonal Antibody (25F2.D10), PerCP
AHA1 단백질을 인식하는 PerCP 결합 단일클론 항체로, Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. 인간, 마우스, 랫트 시료 반응성. Protein G 정제, 보존제 무첨가. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunocytochemistry (ICC/IF) | 1:1,000 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 25F2.D10 |
| Immunogen | Mouse Aha1 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1.59 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64mM phosphate / 2.48mM MES, pH 7.8, with 0.5M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931693 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45239 was sufficient for detection of Aha1 in 10 µg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody. Detects approximately 38 kDa and can run up to 45 kDa on SDS-PAGE.
Target Information
Acts as a co-chaperone of HSP90AA1 (PubMed:29127155). Activates the ATPase activity of HSP90AA1, increasing its chaperone activity. Competes with inhibitory co-chaperones FNIP1 and TSC1 for binding to HSP90AA1, providing a reciprocal regulatory mechanism for chaperoning of client proteins (PubMed:27353360, 29127155). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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