Thermo Fisher Scientific HDEL Monoclonal Antibody (2E7), PE
HDEL 단백질 인식용 Thermo Fisher PE 결합 단클론 항체로, 효모·식물 등에서 HDEL 서열 검출에 적합. Western blot과 면역세포염색에 사용 가능하며, 단백질 G 정제 및 4°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Plant, Yeast |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E7 |
| Immunogen | Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation/Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931697 |
Additional Formats
- Unconjugated (MA5-27549)
- APC (MA5-45240)
- FITC (MA5-45241)
- PerCP (MA5-45242)
- Request custom conjugation
Product Specific Information
1 µg/mL of MA5-45243 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 78 kDa.
Target Information
The HSP70 family comprises four highly conserved proteins — HSP70, HSC70, GRP75, and GRP78 — functioning as molecular chaperones. They assist in multi-protein complex assembly, translocation of polypeptides across membranes, and proper folding of nascent chains.
GRP78 resides in the endoplasmic reticulum (ER), handling secretory proteins and facilitating folding and translocation. Protein sorting depends on a C-terminal tetrapeptide signal, typically KDEL in animal cells and HDEL in S. cerevisiae.
Clone 2E7 recognizes the C-terminal HDEL peptide, a common ER retention signal in yeast, plants, nematodes, and other species. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum, and wheat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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