Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (6C11-B3), FITC
FITC로 표지된 Thermo Fisher Scientific의 Ubiquitin 단클론 항체(6C11-B3)는 Western blot, ICC/IF, ELISA 등에 적합합니다. 모든 종에서 반응하며, 고순도의 Protein G 정제 항체로 4°C 암소 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Bovine, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6C11-B3 |
| Immunogen | Native bovine ubiquitin, conjugated to KLH |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931685 |
Additional Formats
- APC (Cat. No. MA5-45230)
- PerCP (Cat. No. MA5-45232)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45231 was sufficient for detection of ubiquitin in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 10 kDa.
Target Information
Ubiquitin is a highly conserved protein that plays a major role in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. The gene encoding ubiquitin-ribosomal fusion proteins (such as ribosomal protein S27a and L40) produces fusion proteins that are post-translationally processed to generate free ubiquitin monomers and ribosomal components. These ribosomal proteins contain zinc finger domains and are localized in the cytoplasm. Multiple alternatively spliced transcript variants encoding the same proteins have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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