Thermo Fisher Scientific HDEL Monoclonal Antibody (2E7), FITC
HDEL 서열을 인식하는 FITC 결합 단일클론 항체로, 효모 및 식물 단백질의 ER 보유 신호 검출에 적합. Western blot 및 Immunocytochemistry에 사용 가능. 498/517 nm 형광 특성, 단백질 G 정제, 보존제 무첨가.
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Thermo Fisher Scientific HDEL Monoclonal Antibody (2E7), FITC
Applications and Tested Dilution
- Western Blot (WB): 1:1,000
- Immunocytochemistry (ICC/IF): 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Plant, Yeast |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E7 |
| Immunogen | Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip |
| Conjugate | FITC |
| Excitation/Emission Max | 498/517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931695 |
Available Formats
- Unconjugated (MA5-27549)
- APC (MA5-45240)
- PE (MA5-45243)
- PerCP (MA5-45242)
- Request custom conjugation (Thermo Fisher form)
Product Specific Information
- 1 µg/mL of MA5-45241 detects HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 78 kDa.
Target Information
HSP70 family includes HSP70, HSC70, GRP75, and GRP78—highly conserved molecular chaperones involved in protein folding, assembly, and translocation.
GRP78 is localized in the endoplasmic reticulum (ER), aiding in proper folding and transport of nascent peptides.
Protein sorting is determined by C-terminal retention signals (KDEL in animals, HDEL in yeast).
Clone 2E7 specifically recognizes the HDEL peptide, an ER retention motif found in yeast, plants, nematodes, and other eukaryotes.
It stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum, and wheat.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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