Thermo Fisher Scientific Calreticulin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 87 publications 87 publications

Immunohistochemistry (IHC)

Assay-dependent

View 14 publications 14 publications

Immunocytochemistry (ICC/IF)

1:50 - 1:200

View 57 publications 57 publications

Flow Cytometry (Flow)

Assay-dependent

View 12 publications 12 publications

ELISA (ELISA)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

View 13 publications 13 publications

in situ PLA (PLA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rabbit, Rat

Published species

Amphibian, Bovine, Dog, Echinoderm, Hamster, Human, Mouse, Non-human primate, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant, human calreticulin protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325990

Product Specific Information

PA3-900 detects calreticulin from human, non-human primate, rabbit, mouse, canine and rat samples.

PA3-900 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects an ~60 kDa protein representing calreticulin from rat brain extract. Immunofluorescence staining of calreticulin in rat brain cortex with PA3-900 results in fine granular staining consistent with ER localization.

PA3-900 immunogen is recombinant human calreticulin protein produced in the Baculovirus insect cell system.

Target Information

Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.