
Thermo Fisher Scientific CD22 Monoclonal Antibody (OTI1F12), TrueMAB
CD22 단백질을 인식하는 단클론 항체로, Western blot 및 면역세포염색에 적합합니다. Human, Mouse, Rat, Dog 시료에 반응하며, IgG1 아이소타입의 Mouse 유래 항체입니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:4,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F12 |
| Immunogen | Full length human recombinant protein of human CD22 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD22 (BL-CAM) is a type I integral membrane glycoprotein (130–140 kDa) expressed in the cytoplasm and membrane of B-lymphocytes.
It appears early in B-cell differentiation, similar to CD19, and is mainly expressed in mature B cells (CD22+) but not in plasma cells (CD22−).
CD22 is highly expressed in hairy cell leukemia and positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
It binds preferentially to α2,6-linked sialic acid and plays a regulatory role in B cell receptor signaling through tyrosine phosphorylation and interaction with Src family kinases and phosphatases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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