Thermo Fisher Scientific CD22 Monoclonal Antibody (OTI1B1), TrueMAB
인간 CD22 단백질을 인식하는 마우스 단클론 항체로, WB, IHC, ICC/IF에 사용 가능. Lyophilized 형태로 제공되며, 1 mg/mL 농도. B세포 표지자 연구 및 백혈병, 림프종 표지 분석에 적합.
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Thermo Fisher Scientific CD22 Monoclonal Antibody (OTI1B1), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B1 |
| Immunogen | Full-length human recombinant protein of human CD22 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD22 (BL-CAM) is a type 1 integral membrane glycoprotein (130–140 kDa) expressed in both cytoplasm and cell membrane of B-lymphocytes.
It appears early in B-cell differentiation, similar to CD19 antigen.
Membrane expression is limited to late differentiation stages (mature B cells to plasma cells), useful for phenotyping mature leukemias.
CD22 is strongly expressed in hairy cell leukemia, diffuse large B-cell lymphoma, and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
It binds preferentially to α2,6-linked sialic acid and plays roles in regulation of B-cell receptor signaling through phosphorylation and interaction with Src family tyrosine kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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