Thermo Fisher Scientific CD22 Monoclonal Antibody (OTI1F2), TrueMAB
CD22 단백질을 인식하는 마우스 단클론 항체로, Western Blot과 IHC(P) 실험에 적합합니다. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능합니다. 인간 CD22 단백질을 항원으로 제작된 TrueMAB 시리즈 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F2 |
| Immunogen | Full length human recombinant protein of human CD22 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CD22 (BL-CAM) is a type I integral membrane glycoprotein (130–140 kDa) expressed in both cytoplasm and membrane of B-lymphocytes.
It appears early during B-cell differentiation, similar to CD19, and is expressed in mature B cells but absent in plasma cells.
CD22 is strongly expressed in hairy cell leukemia and also positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.
It binds preferentially to α2,6-linked sialic acid and plays a regulatory role in B-cell receptor signaling through tyrosine phosphorylation and interaction with Src family kinases and cytoplasmic phosphatases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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