Thermo Fisher Scientific EGFR Recombinant Rabbit Monoclonal Antibody (BLR252L)
EGFR 단백질을 표적하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, IHC, ICC/IF, Flow, IP 등 다양한 응용에 적합. 인간 및 마우스 반응성, 고순도 액상 형태로 제공되며 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | Assay-dependent |
| Immunoprecipitation (IP) | 6 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR252L |
| Immunogen | Peptide representing a region between residue 1160 and the C-terminus (residue 1210) of Human Epidermal Growth Factor Receptor (EGFR). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
EGFR (Epidermal Growth Factor Receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression of EGFR is associated with multiple cancer types and serves as a therapeutic target for both biological and small molecule drugs.
The EGFR gene is located on chromosome 7 and encodes a transmembrane receptor belonging to the HER/ERbB family (including ERbB2, ERbB3, and ERbB4).
Ligand binding (e.g., EGF, TGF-α) induces receptor dimerization and autophosphorylation of cytoplasmic tyrosine residues.
Overexpression has been observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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