Thermo Fisher Scientific CD86 Recombinant Rabbit Monoclonal Antibody (BLR255L)
Thermo Fisher CD86 Recombinant Rabbit Monoclonal Antibody (BLR255L)은 마우스 CD86 단백질을 표적하는 고특이성 항체로, Western blot, ICC/IF, Flow cytometry, IP에 적합합니다. HEK293F 발현 시스템에서 생산된 정제 항체로 BBS buffer(pH 8.2)에 보관되며 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL/10⁶ cells |
| Immunoprecipitation (IP) | 6 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BLR255L |
| Immunogen | Recombinant protein representing the extracellular domain of mouse T-lymphocyte activation antigen CD86 (residues 24–245) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | BBS, pH 8.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. It acts as a costimulatory molecule in eliciting T-cell help during antigen presentation. Insufficient co-stimulation involving CD86/CD80 can induce tolerance. CD86 is a type I membrane protein belonging to the immunoglobulin superfamily, expressed by antigen-presenting cells, and serves as a ligand for CD28 and CTLA4 on T cells. Binding with CD28 provides a costimulatory signal for T-cell activation, while binding with CTLA4 negatively regulates activation and diminishes immune response. Alternative splicing results in multiple transcript variants encoding different isoforms. CD86 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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